Disable USB ports on Windows PC via Registry With this trick, you can disable access to your USB(Universal Serial Bus) ports on your Windows based PC to prevent people from taking out data without permission or spreading viruses through the use of USB (pen and flash) drives. To use this trick to disable USB ports , follow the steps given below:- Click on Start . Click on Run . If you cannot find RUN, type it in the search box. Type "regedit" without quotes. This will launch the Registry Editor. Navigate to HKEY_LOCAL_MACHINE\SYSTEM\CurrentControlSet\Services\usbstor. In the work area, double click on Start . In the Value Data box, enter 4. Click on OK . Close Registry Editor and refresh your desktop. To re-enable access to your USB ports, enter 3 in the Value Data box in Step 6 . Try it yourself to make your PC invulnerable from data theft and malware which spread through USB ports. This works on Windows XP, Windows Vista, Windows 7 and Windows